When an algorithm-driven microscopy technique developed in 2021 (and able to run on a fraction of the images earlier techniques required) isn’t fast enough, what do you do? At least, that was the solution used by Dushan Wadduwage, John Harvard Distinguished Science Fellow at the FAS Center for Advanced Imaging. They first tried fluorescence microscopy, a century-old technique that relies on fluorescent molecules and light. “The idea is that we use multiple excitation codes, or multiple patterns to excite, and we detect multiple images,” Wadduwage said. Once AI produced reconstructed images that resembled Wadduwage’s ground-truth reference, the researchers used it to capture new images of blood vessels in a mouse brain.